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Project description
Third-cycle subject: Chemistry
The Syrén lab (www.syrenlab.com) is together with Prof. Hjalmar Brismar’s group currently recruiting a joint PhD-student in biophysics. The research area of the PhD-student is centered on studying the impact of water on protein dynamics and catalysis, utilizing interdisciplinary techniques that embrace enzyme design, molecular dynamics simulations and microscopy.
Water molecules are central for the function of all proteins. Surprisingly, our understanding of interactions between water, protein surfaces and ligands/transition states is poorly developed. In this project we hypothesize that spectator water molecules have an even more key role for protein function than previously believed, and that relocation of water networks during enzyme turnover has an impact in enzyme catalysis and motion.
To test this hypothesis, we will use synthetic biology and protein design in concert with state-of-the-art microscopy and spectroscopy. A cornerstone of the project is to affect hydration of protein interiors, and associated water dynamics, by rational enzyme design; a paradigm that remains unresolved in enzyme design today. Here, we will use innovative protein design strategies developed in the lab of Syrén to engineer water networks in two model systems: a terpene cyclase (Subproject 1) and a Na,K-ATPase, (Subproject 2). The overarching goal is to generate enzymes with enhanced catalytic versatility and altered dynamics. Measurement of protein diffusion of generated variants will be performed in vivo using microscopy techniques. We will use GUVs and oocyte based model systems to perform electrophysiological recordings of transport/enzyme activity and spectroscopic recordings based on FCS for single molecule diffusion properties. The position will be placed at the Science for Life Laboratory in Solna and at the Department of fibre and polymer technology.
What we offer
Eligibility
To be admitted to postgraduate education (Chapter 7, 39 § Swedish Higher Education Ordinance), the applicant must have basic eligibility in accordance with either of the following:
Selection
In order to succeed as an doctoral student at KTH you need to be goal oriented and persevering in your work. In the selection of the applicants, the following will be assessed:
Target degree: Doctoral degree
Information regarding admission and employment
Only those who are or have been admitted to third-cycle studies may be employed as a doctoral student. The term of the initial contract may not exceed one year and may thereafter be extended. Doctoral students may engage in teaching, research, and administration corresponding to a maximum of 20 % of a full-time position.
Union representatives
You will find contact information for union representatives on KTH's website.
Doctoral section (Students’ union on KTH Royal Institute of Technology)
You will find contact information for doctoral section on the section's website.
Application
Apply for the position and admission through KTH's recruitment system. It is the applicant’s responsibility to ensure that the application is complete in accordance with the instructions in the advertisement.
Applications must be received at the last closing date at midnight, CET/CEST (Central European Time/entral European Summer Time).
Applications must include the following elements:
Other information
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Disclaimer: In case of discrepancy between the Swedish original and the English translation of the job announcement, the Swedish version takes precedence.
Type of employment | Temporary position |
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Contract type | Full time |
First day of employment | According to the agreement |
Salary | Monthly salary according to KTH's doctoral student salary agreement |
Number of positions | 1 |
Full-time equivalent | 100% |
City | Stockholm |
County | Stockholms län |
Country | Sweden |
Reference number | C-2018-2077 |
Contact |
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Published | 06.Dec.2018 |
Last application date | 28.Feb.2019 11:59 PM CET |